MANI'S MICROBIOLOGY

  TERMINOLOGIES IN MICROSCOPY 1.       Reflection: when a beam of light strikes the smooth surface of a non-transparent material (e.g., the Polished surface of a mirror), it is bounced back, i.e., reflected. The return of light or sound waves from a surface.   2.      Refraction: when a beam of light strikes a smooth interface separating two transparent media (e.g. air and glass; air and water; glass and water), it is partly reflected and partly transmitted, i.e., refracted, into the second medium.                      Reflection and refraction of light would help us in understanding the principles of microscopy. 3.       Lens:             A lens is an optical system with two refracting surfaces. There are two categories of lenses Ø             Convergin...

    DIFFERENCES IN MICROSCOPES Features Compound microscope Phase – contrast microscopes Fluorescence microscopes Electron microscopes Scanning acoustic microscope Source Natural light/ Artificial light Artificial light source UV Light, LASER Electron source Sound waves Types 1.       Bright Field 2.      Dark Field 1.       Phase contrast 2.      Differential interference contrast 1.       Fluorescence 2.      Confocal laser scanning 3.      Two-photon microscope 1.       Scanning electron 2.      Transmission electron 1.       Scanning acoustic micro...