Differences in microscopes (Simplified version)

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DIFFERENCES IN MICROSCOPES

Features

Compound microscope

Phase – contrast microscopes

Fluorescence microscopes

Electron microscopes

Scanning acoustic microscope

Source

Natural light/ Artificial light

Artificial light source

UV Light, LASER

Electron source

Sound waves

Types

1.      Bright Field

2.     Dark Field

1.      Phase contrast

2.     Differential interference contrast

1.      Fluorescence

2.     Confocal laser scanning

3.     Two-photon microscope

1.      Scanning electron

2.     Transmission electron

1.      Scanning acoustic microscope

Cells viewed

Stained, unstained live cells

Unstained live cells

Stained, live cells

Stained, killed cells

Unstained

Images

Coloured (Bright field),

contrast images (dark field)

Dark contrast images

Coloured images

Black and white images, 2D and 3D images

Graphical represented images

Condenser

Bright field;

Dark field

Phase contrast condenser with annular stop.

DIC – two Wollaston prisms

Filters: 1. Exciter filter  

2.     Barrier filter

3.     Dichroic mirror

Confocal: Pinhole apertures

Electromagnetic coils

 

Smear preparation

Thin

Thin

Thin;

thick for Two photon

Thin for TEM

Thick for SEM

Thick and thin

Stains used

Acids and bases

No stain

Fluorochromes

Metals

Water as a medium

 

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